FIGURE 5.

Chrysophanic acid suppresses PPARγ in competition against the PPARγ agonist troglitazone in 3T3-L1 adipocytes. (A) Western blot analyses of PPARγ and C/EBPα were performed and (B) quantification of the protein bands was measured using ImageJ. (C) Western blot analysis of PPARγ was performed under co-treatment of CA with GW or Trog, and (D) quantification of the protein bands was measured using ImageJ. EGCG (10 μM) and GW (10 μM) were used as positive controls. Trog (10 μM) was used as a negative control. GAPDH was used as endogenous control. Data represent means ± SD of three independent experiments. ^#p < 0.05 compared with MDI-uninduced preadipocytes, ^∗p < 0.05 compared with MDI-induced adipocytes. MDI, differentiation medium.