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Characteristics of the human epiphyseal chondrocytes (HECs). (a) Proliferative capacity of HECs. The number of cells was counted at each passage in triplicate independent experiments. Total number of population doubling levels (PDLs) was calculated, using the formula of log10 (total number of cells/starting number of cells)/log102. (b) RT-PCR analysis of HECs #1 for cartilage-associated genes. P: passage number.
