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Primer pairs and experimental conditions for RT-PCR.
| Gene product | Forward and reverse primers (5′-3′) | Expected product size (bp) |
|---|---|---|
| Collagen I | CCCAAGGACAAGAGGCAT | 159 |
| GCAGTGGTAGGTGATGTTCTG | ||
| Collagen II | AGATGGCTGGAGGATTTGAT | 301 |
| CTTGGATAACCTCTGTGACTT | ||
| Collagen III | GGGTCCTACTGGTCCTATTGG | 229 |
| TCGAAGCCGAATTCCTGGTCT | ||
| Collagen X | AGCCAGGGTTGCCAGGACCA | 387 |
| TTTTCCCACTCCAGGAGGGC | ||
| β2-microglobulin | CTCGCGCTACTCTCTCTTTCTGG | 335 |
| GCTTACATGTCTCGATCCCACTTAA |
