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. 2004 Aug 16;32(14):4368–4376. doi: 10.1093/nar/gkh754

Figure 6.

Figure 6

The dimerization motif is located in the KH34 didomain. (A) Western analysis of the ability of endogenous IMP1 from an RNAse T1-treated cytoplasmic extract of RD-cells to associate with His–KH1-4 (track 1), His–KH12 (track 2) or His–KH34 (track 3) on nickel beads. The anti-IMP1 antibody is raised towards the C-terminal 10 amino acids (6), thus being unable to detect His–KH12 in track 2. (B) C-terminally labelled KH1-4 was partially cleaved with either chymotrypsin or LysC and then bound to immobilized GST–KH1-4. The pellet (p) or the supernatant (s) was analysed by polyacrylamide–SDS–tricin gel electrophoresis. The positions of the four KH domains are shown at the left, and amino acid positions and p/s ratios at the right of the autoradiograph.