FIG. 4.

PRDM16, PGC-1α and UCP1 expression after acute exercise.

Total RNA from eWAT and iWAT was isolated and the expression of PRDM16 (A), PGC-1α (B), UCP1 (C), TH (F) and Dio2 (H) was assessed by RT-PCR. Total RNA from SVF and adipocytes from eWAT were isolated and the expression of perilipin (D) and UCP1 (E) was assessed by RT-PCR. *P<0.05, **P<0.01, #P<0.001 vs. Sed. After 2 h of cold exposure (4°C), total RNA from eWAT and iWAT was isolated and the expression of TH (G), Dio2 (I), PRDM16 (J), PGC-1α (K) and UCP1 (L) was assessed by RT-PCR. Each value was normalized to cyclophilin A. Bars represent the means and error bars represent SD (N=8). *P<0.05, #P<0.001 vs. RT.