Figure 1.

RRM2 of SF2/ASF is necessary and sufficient for the recruitment to nSBs. (A) Scheme of the SF2/ASF protein and of the different deletion mutants. Numbers on the right indicate the amino acid regions present in each mutant. (B) The entire SF2/ASF or the indicated deletion mutants shown in (A) were overexpressed as GFP fusions in HeLa cells. After 40 h, the cells were heat shocked (for 1 h at 42°C followed by 1 h at 37°C) and immediately fixed. Cells were stained with a polyclonal antibody to hnRNP HAP and with anti-rabbit rhodamine-conjugated goat antibody. Cells were analyzed by confocal laser microscopy to study the distribution of the GFP protein and of hnRNP HAP. Images of the same cells were taken and merged to reveal co-localization of the two proteins in nSBs.