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. 2004 Aug 9;32(14):4127–4136. doi: 10.1093/nar/gkh759

Figure 2.

Figure 2

Effect of amino acid substitutions in RRM2 on the recruitment of SF2/ASF to the nSBs. (A) Alignment of the amino acid sequence of RRM2 in three SR proteins recruited to nSBs. The position of RNP-1 and RNP-2 motif is indicated along with their consensus sequence. At the bottom is shown the consensus of the three sequences and the position of the α-helices and β-sheets. Mutated amino acids are indicated in the boxes above the first line. (B) SF2/ASF mutants bearing the indicated amino acid substitutions in RRM2 were overexpressed in HeLa cells as GFP fusions. After 40 h, the cells were heat shocked as in Figure 1B, fixed and stained with a polyclonal antibody to hnRNP HAP and with anti-rabbit rhodamine-conjugate goat antibody. The cells were analyzed by confocal laser microscopy to study the distribution of the GFP proteins and of hnRNP HAP. Images of the same cells were taken and merged to reveal co-localization of the two proteins in nSBs.