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. 2016 Dec 8;6:38275. doi: 10.1038/srep38275

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Copyright © 2016, The Author(s)

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Trem1 transcript expression visualized by using in situ hybridization (ISH) in sham and 1 day after bilateral IR. Lines in sham and IR (4× magnification) indicate the cortico-medullary (CM) area. Sham pictures (40×) shows an inset with 100× magnification. From the IR (40×) picture we selected two areas (I and II) to show in higher magnification (100×). Original magnification 4×, 40× and 100× (A). Intrarenal Trem1 transcript quantified by RT-PCR (n = 6–7/group) (B) and protein expression as determined by western blot (n = 3) and ELISA (n = 6–7/group) (C,D). Values are expressed as mean ± SEM. *P < 0.05 vs sham; **P < 0.005 versus sham.