Figure 2.

Cleavage of plasmid and catenane substrates by EcoP15I. The outcome of single- or double-strand cleavage of generic plasmid (a) or catenane (b) substrates is shown, alongside the corresponding acronyms for each fragment. Dimeric forms of the plasmid (Dimer) and the large and small rings of the catenane (Dimer_L and Dimer_S, respectively) are not illustrated. (c) Cleavage of pMDS35a and 35acat. (d) Cleavage of pMDS34a and 34acat. In each case, 10 nM DNA was incubated for 1 h with EcoP15I, SmaI or PstI as indicated (by a black circle) and the resulting fragments separated by agarose gel electrophoresis (see Materials and Methods and text for further details). Markers for the plasmid and catenane fragments are on the left and right, respectively, of each gel. Gel images were adjusted to maximize fragment brightness, for the purposes of visualization only, using a linear intensity scale.