Table 4. Complementation analysis of scm mutations.
| Frequency of aberrant asci from cross (%) | ||||||||
|---|---|---|---|---|---|---|---|---|
| Genotype | WSP # | Wild-type 0607 | rec121813 | scm11892 | scm21904 | scm31916 | scm41927 | scm51938 |
| Wild-type | 0620 | 2 | 3 | 1 | 4 | 6 | 3 | 2 |
| rec12 | 1827 | 4 | 54 | 3 | 5 | 3 | 6 | 2 |
| scm1 | 1890 | 2 | 5 | 32 | 0 | 1 | 3 | 1 |
| scm2 | 1902 | 1 | 3 | 2 | 26 | 2 | 1 | 1 |
| scm3 | 1914 | 1 | 3 | 3 | 1 | 29 | 6 | 0 |
| scm4 | 1925 | 1 | 4 | 3 | 2 | 3 | 48 | 5 |
| scm5 | 1936 | 3 | 2 | 2 | 0 | 1 | 0 | 35 |
Haploid strains of mating type h− (top row) and mating type h+ (left column) were mixed and plated on sporulation agar to induce mating, meiosis and ascus formation. At least 100 asci from each cross were examined by DIC and DAPI microscopy as in Figure 3 to determine the frequency of asci with aberrant chromosome partitioning.