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. 2004 Aug 10;32(14):4297–4305. doi: 10.1093/nar/gkh769

Figure 1.

Figure 1

Promoter structure of wild type and mutant alleles of SNR6. The wild-type S.cerevisiae U6 RNA gene, SNR6, is shown schematically at the top. The open box represents U6 RNA coding sequence (+1 to +112) and black boxes represent promoter elements. The SPIN+B allele was created by inserting the 50 bp S.pombe U6 intron, the non-template strand sequence of which is shown, between positions +58 and +59 of SNR6. The consensus splice elements and core B block motif of the intron are underlined. (Note that the fourth intron nucleotide was mutated from A to T to match the S.cerevisiae 5′ splice site consensus.) The SPIN−B allele is truncated at position +235 (12). The SPIN alleles were modified by replacing 16 bp of the S.pombe U6 intron with 26 bp (+228 to +253) encompassing the SNR6 downstream B block (SCIN), or by addition of 9 bp between the branchpoint and 3′ splice site (+9). In a separate construct (SpDsB), SNR6 sequences +228 to +253 were replaced with the indicated sequence from the S.pombe intron.