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. 2004 Aug 10;32(14):4313–4321. doi: 10.1093/nar/gkh771

Figure 8.

Figure 8

UV1 and UV2 repeats in the RAD51 promoter are responsible for proper expression during conjugation. (A) Reporter constructs in transformants expressing GFP from the full-length, wild-type promoter (RdGFP), two promoter truncations [RdGFP (−316Δ) and RdGFP(−182Δ)], and the full-length promoter with mutated UV1 and UV2 repeats [RdGFP(UV1+2)] are shown schematically as in Figures 1 and 4 for the RdLuc reporter. (B) GFP mRNA was monitored during the first 7 h of conjugation between transformant clonal lines. Each conjugation was repeated in triplicate. A northern blot of total RNA (10 μg per lane), isolated from each time point and hybridized with a GFP-specific radiolabeled probe, was quantified by PhosphorImager analysis. GFP mRNA levels are indicated by light grey bars for RdGFP, black bars for RdGFP(−316Δ), white bars for RdGFP(−182Δ) and dark grey bars for RdGFP(UV1+2). Error bars represent the standard deviation.