Table 1. Tetrahymena thermophila strains.
| Strain | Micronuclear genotype | Macronuclear genotype | Macronuclear phenotype |
|---|---|---|---|
| CU522 | mpr1/mpr1, btu1-1/btu1-1 | mpr1, btu1-1 | mp-R, ory-R, pac-S, VI |
| CU725 | chx1/chx1, btu1-1/btu1-1 | chx1, btu1-1 | cy-R, ory-R, pac-S, VII |
| TC202 | mpr1/mpr1, btu1-1/btu1-1 | mpr1, btu1-1::Luc | mp-R, ory-S, pac-R, VI BtLuc |
| TC232 | mpr1/mpr1, btu1-1/btu1-1 | mpr1, btu1-1::RdLuc | mp-R, ory-S, pac-R, VI RdLuc |
| TC296 | chx1/chx1, btu1-1/btu1-1 | chx1, btu1-1::RdLuc | cy-R, ory-S, pac-R, VII RdLuc |
| TC368 | mpr1/mpr1, btu1-1/btu1-1 | mpr1, btu1-1::RdGFP | mp-R, ory-S, pac-R, VI RdGFP |
| TC370 | chx1/chx1, btu1-1/btu1-1 | chx1, btu1-1::RdGFP | cy-R, ory-S, pac-R, VII RdGFP |
chx1, cycloheximide resistance (cy-R); mpr1, 6-methylpurine resistance (mp-R); btu1-1, paclitaxel sensitivity (pac-S) and oryzalin resistance (ory-R). BtLuc, luciferase expressed from the BTU1 promoter; RdLuc, luciferase expressed from the RAD51 promoter; RdGFP, GFP expressed from the RAD51 promoter. The genotypes and phenotypes of representative transgenic strains are listed in this table. Transgenic strains not listed include those expressing reporter genes from variously mutated promoters described in the text.