Western blots for P-glycoprotein expression. After treating NIH 3T3 MDR cells with various antisense/Lipofectamine 2000 complexes as described, cells were harvested for western analysis. Lane 1, Lipofectamine 2000 only control; lane 2, HNA gapmer (GS1956); lane 3, phosphorothioate (5995); lane 4, 2′-O-ME gapmer (13 758); lane 5, HNA gapmer mismatch (GS1957); lane 6, phosphorothioate scrambled (10 221); lane 7, 2′-O-ME gapmer scrambled (13 753). All oligonucleotides were used at a 10 nM concentration.