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. 2004 Aug 10;32(14):e115. doi: 10.1093/nar/gnh110

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Agarose gel electrophoresis of mutagenesis reactions. Lane M: 1 kb DNA ladder standard (Fermentas). Lanes 1–4 use template pBAD-14D9: lane 1, D_H101A^*; lane 2, D_H101A; lane 3, A_L46T; and lane 4, F_H98A. Lanes 5–8 use template pBAD-10F11: lane 5, W_H104Y^*; lane 6, W_H104Y; lane 7, S_H100A; and lane 8, L_L101F. Lanes 9–11 use template pBAD-16E7: lane 9, D_H50A; lane 10, E_L39A; and lane 11, S_H99A; lane 12, F_H98A with template pQE-14D9ScFv; lane 13, F_H98A with template pET-14D9ScFv-NusA; and lane 14, CMV.