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Agarose gel electrophoresis of site-directed saturation mutagenesis reactions. Lane M: 1 kb DNA ladder standard (Fermentas); lane 1, randomized PCR SSM1 with annealing temperature of 52°C; lane 2, randomized PCR SSM1 with annealing temperature of 57°C; lane 3, randomized PCR SSM2 with annealing temperature of 52°C; and randomized PCR SSM2 with annealing temperature of 57°C.
