Table 2. Randomization frequency of each nucleotide at H98 position.

	PCR product (%)				Randomized library (%)
	G	C	A	T	G	C	A	T
Position M	—	53.7	46.3	—	34.3	40	25.7	—
Position N1	10	37.1	28.6	24.3	12	15.7	54.2	18.1
Position N2	20.7	35.4	24.4	19.5	11.7	18.1	52.1	18.1

Occurrence frequencies were calculated according to signal amplitude at each position from sequencing chromatography.