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. 2016 Dec 8;6:38498. doi: 10.1038/srep38498

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Copyright © 2016, The Author(s)

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(a) TEM results showing spherical exosomes isolated from hAMSC-CM. (b) Bar diagram representing the size distribution of exosomes ranging between 50 nm and 150 nm in diameter, with the majority between 70 nm and 100 nm in diameter. (c) Coommassie Blue staining of SDS-PAGE results indicating protein distributions in exosome and cell lysates. (d) Expression of exosome-specific marker (α-CD63) in exosome-derived proteins detected by western blot. (e) Internalization of glow-exosomes (fluorescence-tagged exosomes) by A2780 cells. (f) Representative photo of wound-healing assay. Exosome-treated cells exhibited decreased wound-healing capacity as compared with non-treated control cells. (g) Colony formation ability of exosome-treated cells declined as compared to non-treated control cells. (h) The percentage plating efficiency decreased significantly (p < 0.05) following exosome treatment as compared with control cells. (i) The percentage survival fraction decreased significantly (p < 0.01) in cells treated with hAMSC-CM-derived exosomes as compared with non-treated controls. (j) Cell viability of A2780 cells reduced significantly (p < 0.01) following exosome treatment. (k) The percentage cell viability continued to decrease (p < 0.01) following treatment with protease-digested exosome lysate as compared with controls. (l) The percentage cell viability in cells treated with RNase-digested exosome lysate did not decline, but rather resulted in slight increases in viability. The experiments were repeated at least three times. *p < 0.05 and **p < 0.01.