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. 2016 Dec 8;6:38498. doi: 10.1038/srep38498

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) Analysis of freshly isolated exosomal RNA indicated no peaks detected at 18 S or 28 S positions; however, exosomal RNA samples were enriched with small RNA. (b) Analysis showing preparation of the small-RNA library from exosomal RNA. (c) Sequence-quality data for exosomal RNA. (d) Pie chart illustrating the overall composition of exosomal RNA, with ~2% known miRNAs and 1.2% potential candidate miRNAs. (e) Venn diagram showing the distribution of known miRNA detected in three different replication samples and the 141 miRNAs common among all three samples. (f) Venn diagram representing the distribution of potential candidate miRNA in different samples and the 25 miRNAs common among all three samples. The experiments were repeated at least three times.