Table 2. Number of cII mutations (%) detected in liver tissues from PFOS exposed λ transgenic medaka.
| Mutation Spectrum | PFOS (μg/L) | |||
|---|---|---|---|---|
| 0a | 6.7b | 27.6a,c | 87.6b,c | |
| Total mutations | 46 | 49 | 48 | 44 |
| Independent mutations | 33 | 36 | 38 | 41 |
| Transition | 14 (42.4) | 8 (22.2) | 11 (28.9) | 9 (22.0) |
| G/C→A/T | 6 (18.2) | 4 (11.1) | 8 (21.1) | 6(14.6) |
| A/T→G/C | 8 (24.2) | 4 (11.1) | 3(7.9) | 3(7.3) |
| Transversion | 15 (45.5) | 23 (63.9) | 20 (55.3) | 20 (48.8) |
| G/C→T/A | 5 (15.2) | 10 (27.8) | 12 (31.6) | 8 (19.5) |
| G/C→C/G | 3 (9.1) | 11 (30.6) | 3 (7.9) | 4 (9.8) |
| T/A→A/T | 1 (3.0) | 0 (0.0) | 3 (7.9) | 5 (12.2) |
| A/T→C/G | 6 (18.2) | 2 (5.6) | 2 (5.3) | 3 (7.3) |
| Frameshift | 3 (9.1) | 2 (5.6) | 7 (18.4) | 10 (24.4) |
| +1 | 0 (0.0) | 2 (5.6) | 5 (13.2) | 6 (14.6) |
| −1 | 3 (9.1) | 0 (0.0) | 2 (5.3) | 1 (2.4) |
| Indel* | 0 (0.0) | 0 (0.0) | 0 (0) | 3 (7.3) |
| Other** | 1 (3.0) | 3 (8.3) | 0 (0.0) | 2 (4.9) |
a,b,cTreatment groups sharing the same superscript letter are not significant different from each other based on the Monte Carlo method of Adams and Skopek.
*Frameshift caused by multi-base insertion and deletion.
**Multiplex single base substitutions at different nucleotides.
Values underlined indicate significant difference from the control group based on the Pearson’s chi-squared test (P < 0.05).