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. 2016 Dec 8;6:38788. doi: 10.1038/srep38788

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Copyright © 2016, The Author(s)

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(A) Results from HS-YFP assay carried out on HEK-293 cells expressing the indicated constructs. Gray bars: anion transport in the absence of intracellular Ca²⁺ elevation. White bars: anion transport after stimulation with ionomycin (1 μM). ^#p < 0.05 vs. C-TERM1 (n = 6–9 experiments). (B) Representative traces from HS-YFP experiments. (C) Representative whole-cell membrane currents and current-voltage relationships from 6–9 experiments with the indicated TMEM16A constructs. Intracellular free Ca²⁺ concentration was 7 or 245 nM.