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. 2016 Dec 8;6:38750. doi: 10.1038/srep38750

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(a) HOC313-P cells were treated with PBS or LM-exosomes, and the effect of exosomes on cell growth was determined using an in vitro WST-8 assay at the indicated times. (b) Western blot analysis of EGFR, ERK and AKT and their phosphorylation status is shown in HOC313-P cells treated with LM-exosomes for the indicated amounts of time. (c and d) Cell migration was assessed by Transwell migration assay (c) and cell invasion was assessed by Transwell invasion assay (d) in HOC313-P cells treated with either PBS or LM-exosomes. Experiments were performed in triplicate. (Bars, SD). Student’s t-test was used for statistical analysis; asterisks represent P < 0.05 versus each control transfectant.