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. 2016 Dec 8;6:38750. doi: 10.1038/srep38750

Figure 3. LM-exosomes contain oncogenic miRNAs and mediate their transfer to promote intercellular communication.

Figure 3

(a) The Venn diagram illustrates the overlapping result of differentially expressed miRNAs in whole cells and exosomes, including HOC313-P and HOC313-LM cell comparisons. Two miRNAs, miR-342-3p and miR-1246, were upregulated in both whole cells and exosomes. (b and c) MiR-342-3p and miR-1246 were transferred to HOC313-P cells treated with LM-exosomes (b) or across a Transwell membrane through exosomes (c). (d) Validation of miR-342-3p and miR-1246 expression after each miRNA transfection in HOC313-P cells was measured by qRT-PCR. MicroRNA expression levels were normalized to RNU6B expression. MicroRNA expression levels of miR-342-3p (left) and miR-1246 (right) relative to the negative controls are shown. (e) Cell growth after 72 hours of transfection with miR-342-3p, miR-1246 and miR-NC in HOC313-P cells was evaluated by WST-8 assay. (f and g) Cell motility was assessed by Transwell migration assay (f) and cell invasion was assessed by Transwell invasion assay (g) in HOC313-P cells transfected with miR-342-3p or miR-1246. Experiments were performed in triplicate. (Bars, SD). Student’s t-test was used for statistical analysis; asterisks represent P < 0.05 versus each control transfectant.