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. 2016 Dec 8;6:38689. doi: 10.1038/srep38689

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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Representative flow cytometry histograms showing intracellular TNF accumulation in (a) monocytes and (b) lymphocytes after incubation with PHA for 16 hours. Dark grey line, unstimulated isotype control antibody; light grey, unstimulated TNF; solid black, PHA stimulated TNF. x axis, TNF fluorescence intensity; y axis, number of events. (c) Intracellular TNF accumulation in monocytes in response to 10 μg/ml PHA in patients with sarcoidosis and controls (n = 3). Results are presented as mean ± SEM geometric mean fluorescence intensity; *p < 0.05, **p < 0.01 using two-way ANOVA with Bonferroni’s post hoc test.