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. 2016 Dec 8;6:38697. doi: 10.1038/srep38697

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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(A) The venn diagram shows genes that are upregulated in the db/db white adipose tissue and up-regulated in 3T3-L1 adipocytes by the interaction with activated RAW264.7 macrophages. Of a total 402 genes up-regulated in db/db adipose tissue, the expression of 15 genes was also increased in 3T3-L1 adipocytes by the interaction with activated macrophages (p < 0.05). (B) Total RNAs from several types of tissue of db/+ and db/db mice were isolated. The relative mRNA expression level of each gene was determined by quantitative PCR and normalized to L19 mRNA level. The data are representative of two independent experiments.