Table 1. Analysis of two transcriptomes to isolate genes whose expression is upregulated by the interaction with macrophages.

GeneName	Accession No.	Description	Fold 1 (db/db)	Fold 2 (co-culture)
Hmga2	NM_010441	high mobility group AT-hook 2	38.0	5.0
Il1rn	NM_031167	interleukin 1 receptor antagonist, transcript variant 1	31.8	14.2
Cxcl5	NM_009141	chemokine (C-X-C motif) ligand 5	12.1	22.2
Bub1	NM_009772	budding uninhibited by benzimidazoles 1 homolog (S. cerevisiae)	10.4	92.4
Il1rl1	NM_010743	interleukin 1 receptor-like 1, transcript variant 2	10.3	6.7
Serpinb2	NM_011111	serine (or cysteine) peptidase inhibitor, clade B, member 2	10.1	29.5
Bst1	NM_009763	bone marrow stromal cell antigen 1	9.2	8.8
Timp1	NM_011593	tissue inhibitor of metalloproteinase 1, transcript variant 2	9.2	8.4
Steap1	NM_027399	six transmembrane epithelial antigen of the prostate 1	7.4	6.1
Saa3	NM_011315	serum amyloid A 3	6.3	72.7
Rasgrf1	NM_001039655	RAS protein-specific guanine nucleotide-releasing factor 1	6.2	9.4
Slc15a3	NM_023044	solute carrier family 15, member 3	6.1	28.7
Clec4e	NM_019948	C-type lectin domain family 4, member e	6.1	7.4
Mmp3	NM_010809	matrix metallopeptidase 3	6.1	41.6
Lcn2	NM_008491	lipocalin 2	5.6	16.7

DNA microarray analysis was repeated with the Cy3 and Cy5 dyes reversed (a dye swap). Fold change (Fold 1) represents the average of mRNA expression level in db/db mice relative to db/+ mice. Fold change (Fold 2) represents the average of mRNA expression level in the 3T3-L1 adipocytes upon co-culture with macrophages in the presence of LPS relative to with macrophages in the absence of LPS.