Figure 3. Mice vaccinated in early life can handle both acute and chronic viral infection as efficiently as mice primed as adults.
C57BL/6 infant mice were vaccinated with 2 × 106 or 2 × 107 pfu Ad-liGP s.c in the foot pad and 60 days later challenged i.p with 2 × 105 pfu LCMV Armstrong (A), 2 × 105 pfu LCMV Clone 13 (B) or 2 × 106 pfu LCMV Clone 13 (C). Mice, vaccinated as adults with 2 × 107 pfu Ad-liGP (ctrl), and naive unvaccinated mice were similarly challenged and used as positive and negative controls, respectively. Three days (A), 5 (B) or 10 (C) days post challenge, viral titers in the spleen were measured by plaque assay. Each dot represents an individual animal. The depicted data are pooled from two independent experiments. The detection limit for virus was determined as 250 pfu/g of spleen. *p < 0.05.