Figure 5. Similar kinetics of the primary GP33 specific CD8+ T-cell response in Ad-liGP-vaccinated mice regardless of age at vaccination.

Groups of C57BL/6 infants and adult mice were vaccinated with 2 × 10⁷ pfu Ad-liGP s.c in the foot pad, and on the indicated days following vaccination, spleens were harvested and IFN-Y producing CD8+ T cells in spleen were enumerated by flow cytometry following ex vivo peptide stimulation with GP_33-41. For each time point 5 animals/group of mice vaccinated as infants and 2 animals/group of mice vaccinated as adults were analyzed. (A) Representative dot plots for the CD8+ T-cell population and IFN-Y producing cells for days 9, 12, 15 post vaccination. (B) absolute numbers of IFN-γ producing CD8+ T cells in the spleen. The results represent the group medians with ranges and are representative of two independent experiments. *p < 0.05.