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. 2016 Dec 8;6:38681. doi: 10.1038/srep38681

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Copyright © 2016, The Author(s)

This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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COS-7 cells treated with either DMSO (a,c,e) or 5 μM MG132 (b,d,f) for 24 h were immunostained with rabbit anti-Cav1 polyclonal antibody and vimentin (a,b), ubiquitin (c,d), or VCP/p97 (e,f). Images were acquired using similar microscope settings for DMSO- and MG132-treated cells. Representative images from 2 independent experiments are shown. In the merged images Cav1 staining is shown in green and vimentin, ubiquitin or VCP/97 staining is depicted in red. DRAQ5 staining was used to label the nucleus (blue). White boxes indicate the areas of the zooms. Scale bars, 10 μm.