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. Author manuscript; available in PMC: 2017 Oct 17.
Published in final edited form as: Chem Res Toxicol. 2016 Sep 13;29(10):1651–1661. doi: 10.1021/acs.chemrestox.6b00186

Figure 5.

Figure 5

Mammalian two-hybrid analysis of MEHP, MiNP, and MBzP activation of CAR, PXR, PPARα, or PPARγ. COS-1 cells were transfected with the ligand binding domains of CAR, PXR, PPARα, or PPARγ in the pm (GAL4) vector, SRC1 in the VP16 vector, pcDNA3.1(+)-RXRα-LBD, pFR-luciferase reporter, and pRL-CMV vector for normalization. Chemical treatments were for 24 h. Postive controls were 3 μM CITCO for CAR2, 0.1 μM TO for PXR, 50 μM WY14643 for PPARα, and 30 μM TG for PPARγ. Data are represented as normalized luciferase values, and each data point represents the mean (±SD) of triplicate treatment wells from a representative transfection experiment.