Table 2. scansite prediction at highest stringency (0.2%) and medium stringency (1.0%) for kinase phosphorylation and binding motifs from this data set.
| Type | Hits (0.2%) | Hits (1.0%) | |
|---|---|---|---|
| Kinase | |||
| Casein kinase 2 | Acidiphilic | 65 | 172 |
| Glycogen synthase kinase 3 | Pro-directed | 64 | 206 |
| CDC2 | Pro-directed | 55 | 262 |
| AKT | Basophilic | 53 | 122 |
| Extracellular signal-regulated kinase 1 | Pro-directed | 51 | 235 |
| Cyclin-dependent kinase 5 | Pro-directed | 49 | 260 |
| P38 mitogen-activated protein kinase | Pro-directed | 33 | 160 |
| PKA | Basophilic | 17 | 48 |
| Clk2 | Basophilic | 11 | 72 |
| DNA-dependent protein kinase | Gln-directed | 8 | 62 |
| Calmodulin-dependent kinase 2 | Basophilic | 7 | 21 |
| Ataxia telangiectasia mutated kinase | Gln-directed | 6 | 23 |
| PKC-δ | Basophilic | 2 | 9 |
| PKC-α/β/γ | Basophilic | 1 | 7 |
| PKC-ε | Basophilic | 1 | 8 |
| Casein kinase 1 | Other | 0 | 23 |
| Protein kinase D | Basophilic | 0 | 5 |
| Motif | |||
| 14-3-3 binding motif | Pro-directed | 31 | 85 |
| Phosphoinositide-dependent kinase 1 binding motif | Pro-directed | 2 | 3 |