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TAP-tagged Imds copurify with untagged Imds. TAP-tagged proteins were isolated on an IgG-containing matrix from strains DY441, DY1100, DY1101, DY419, and DY1103 (left to right), resolved by polyacrylamide gel electrophoresis, and stained with Coomassie blue. The polypeptide at ≈100 kDa in some samples is a contaminant derived from the affinity matrix.
