Fig. 4.

Modification of purified yeast IMPDH in vitro. Affinity-purified TAP-Imd2 and TAP-Imd3 were purified from DY423, DY1103, and DY1112 (Left, Center, and Right, respectively) and incubated alone or in complete reactions with IMP, NAD⁺, and MPA. Two micrograms of each protein were separated by polyacrylamide gel electrophoresis and stained with Coomassie blue.