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. 2016 Dec 8;3(6):ENEURO.0108-16.2016. doi: 10.1523/ENEURO.0108-16.2016

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Copyright © 2016 Khanday et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International, which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Figure 4. — Percentage (±SEM) of total recording time spent in waking state, NREMS, and REMS 8 d after TH-shRNA or Ctrl-shRNA microinjection into the LC and Sal or NA microinjection into the PPT (on the day of recording), and 21 d after TH-shRNA microinjection. a–c, The histograms show analyses of total recording periods of 12 h (10:00 A.M. to 10:00 P.M.; a), 8 h (10:00 A.M. to 6:00 P.M.; b), and 4 h (6:00 to 10:00 P.M.; c). Abbreviations are as in the text. *Compared with baseline values and Ctrl-shRNA injection; $compared with TH-shRNA injection into LC and NA injection into PPT or 3 weeks after the injection of TH-shRNA into LC. Significance level: ***p < 0.001; **p < 0.01; *p < 0.05; N = 6.