Figure 4.

Postnatal microglia depletion impairs nest-seeking behavior and decreases USV emission. Quantification of data from the two-choice nest-seeking task represented as average score per day (a), average total score summed across all testing days (b), and average latency (in seconds) to reach either goal arm of the testing apparatus (c). d, Quantification of the number of USVs produced by pups when separated from the mother on PN8. Solid lines and bars indicate group means for VEH-treated (black) and LC-treated (green) rats of both sexes. Shaded regions and error bars indicate ± SEM. Open circles represent data from individual rats. Horizontal dashed line indicates score equal to chance (a, b). ***p < 0.001. n = 18–20 (a–c) and 13–14 (d) per treatment.