Table 2. Rate of homologous integration at the mtr locus in the wild type and in mus-51, mus-52, mei-3, and mus-11 mutant strains.
| Strains | Exp. no. | Bla-resist | PFP-resist | Homologous integration, % |
|---|---|---|---|---|
| Wild type | 1 | 9 | 3 | |
| 2 | 22 | 3 | ||
| 3 | 11 | 3 | ||
| 4 | 16 | 3 | ||
| Total | 58 | 12 | 21 | |
| mus-51 | 1 | 41 | 41 | |
| 2 | 18 | 18 | ||
| Total | 59 | 59 | 100 | |
| mus-52 | 1 | 12 | 12 | |
| 2 | 23 | 23 | ||
| 3 | 12 | 12 | ||
| 4 | 26 | 26 | ||
| Total | 73 | 73 | 100 | |
| mei-3 | 1 | 58 | 2 | |
| 2 | 35 | 1 | ||
| Total | 93 | 3 | 3* | |
| mus-11 | 1 | 45 | 0 | |
| 2 | 20 | 0 | ||
| Total | 65 | 0 | 0 |
Bla-resist, blastcitidin resistance, PFP-resist, PFP resistance; homologous integration rate = number of PFP-resist/number of Bla-resist.
*
Confirmation of homologous integration was not obtained by PCR