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Cytokine secretion after stimulation of Vα14i NKT cells with glycolipid antigens in vivo. Two (♦) and 14 h (▪) after i.p. injection with 2 μg of antigen, serum was harvested and analyzed for IL-4 (A) and IFN-γ (B) by ELISA. Each point represents the cytokine measured in an individual mouse. The dashed line indicates the limit of detection of the ELISA. There were three mice in each group, and data are representative of two separate experiments.
