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. 2004 Aug 10;101(33):12254–12259. doi: 10.1073/pnas.0404632101

Fig. 4.

Fig. 4.

Specific CD1d tetramer staining. Binding isotherm of α-GalCer-loaded CD1d tetramer (▴), α-GlcCer-loaded CD1d tetramer (•), and 4-deoxy-loaded CD1d tetramer (▪) to the 2C12 hybridoma. Tetramer fluorescence, normalized to the surface level of TCRβ staining, is plotted against tetramer concentration. 2C12 hybridoma cells were stained for3hat room temperature with an anti-TCRβ mAb and the indicated concentrations of WT CD1d tetramers. Cells were then washed two times and analyzed by flow cytometry. One representative experiment of five is shown.