Fig. 1.

Tetramer staining of CD8⁺ T cells. CD8⁺ T cells from spleens of B6 mice and B6.UMT mice were stained with D^b-GagL tetramers. The CD4^- group was depleted of CD4⁺ T cells before immunization with FBL-3 tumor cells. Statistical analysis by using ANOVA with a Tukey–Kramer multiple-comparisons posttest showed significant differences between naive and vaccinated mice of both strains (P < 0.01) and also between FBL-3-immunized B6 and B6.UMT mice (P < 0.05). There were no significant differences between naive and CD4-depleted, FBL-3-immunized mice. Each circle represents results from a single mouse.