Fig. 4.
PrPres accumulation in purified astrocytes and neuron/astrocyte cocultures as assessed by Western blotting. (A) PrPres kinetics in ScCAS338 cultures exposed to ScMov culture medium. (B) Purified CGN338 neurons (99%) grown on top of a confluent nonpermissive CAS0/0 culture and exposed to 0.01% tg338 infectious brain homogenate. Duplicate experiments at 7 and 14 days p.e. show a de novo PrPres synthesis in neurons. PrPres was revealed by PK treatment of 40 μg of protein using mAb ICSM18.