Table 1.
Default threshold values in the gigasealing algorithm
| Parameter | Value | Factor Contributing to Variation |
|---|---|---|
| Rp threshold 1: initial pipette resistance | <10 MΩ | Pipette tip diameter, clogging |
| P(+) threshold 1: minimum positive pressure | >30 mmHg | Extracellular matrix composition, target cell depth (higher pressure may be necessary for deeper cells) |
| Rm threshold 2: touch cell resistance coefficient | >115% of initial Rp | Cell size/type |
| P(−) threshold 1: negative pressure for sealing | Less than −60 mmHg | Cell size/type |
| Rm threshold 3: for −70-mV adjustment | 100 MΩ | Based on experience; may be optimized by data mining |
| Rm threshold 4: wait for gigasealing resistance | 200 MΩ | Based on experience; may be optimized by data mining |
| Rm threshold 5: gigaseal resistance | 1,000 MΩ | Patch quality requirement; a higher value will result in the tighter seal |
| P(−) threshold 2: minimum negative pressure for break-in | Less than −85 mmHg | Cell size/type, ±zapping |
| Rm threshold 6: broken-in resistance | <300 MΩ | Cell size/type |
| Ihold threshold 1: broken-in holding current | Greater than −200 pA and <100 pA | Seal quality |
| t threshold 1: time to fail | 4 min | Based on experience |
Listed values are those used in the experiments described in this article. All listed thresholds are related to the representative patch in Fig. 4, and all can be changed by the user and saved in the Autopatcher IG configuration file.