Figure 5. Complementation of the yeast ost2 knockout strain with the B. braunii DAD1 cDNA.

Serial dilutions (10⁻¹ –10⁻⁶) of yeast cells were plated in SD—URA medium containing 1 mg mL⁻¹ tunicamycin and incubated at 28°C for 48 h (A). Viability of wild type and Δ ost2 (OST2x Δ ost2::KanMX) was calculated after counting number of colonies in the presence or absence of tunicamycin (B). Mean ± SE, n = 3.