Skip to main content
. 2004 Aug 10;101(33):12312–12317. doi: 10.1073/pnas.0404728101

Fig. 1.

Fig. 1.

Plasmids used for transposon mutagenesis in S. aureus. (A) Bursa aurealis, a minimariner transposable element, was cloned into pTS2, with a temperature-sensitive plasmid replicon (repts) and chloramphenicol resistance gene (cat) to generate pBursa; the 3.2-kb bursa aurealis encompasses the mariner terminal inverted repeats (TIR), the green fluorescent protein gene (gfp), the R6K replication origin (oriV), and the erythromycin-resistance determinant (ermB). The position of restriction enzyme recognition sites (AciI and BamHI) is indicated. (B) Plasmid pFA545 encodes the mariner transposase (tnp), the origin of replication (repC), and the tetracycline- and ampicillin-resistance markers (tetBD and bla, respectively). (C) The structure of the 5.3-kb transposon Tn917 with the ermB erythromycin-resistance determinant, tnpR resolvase, and tnpA transposase is shown.