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. 2016 Nov 17;13:154–163. doi: 10.1016/j.nicl.2016.11.015

Fig. 1.

Fig. 1

The Anatomy of the SN on serial axial sections using Luxol fast blue/Cresyl Violet (LFB/CV), Perls stain, high field spin-echo MRI, and substance P (SP) and calbindin (CB) immunohistochemistry. Case 2 was a 36 yr old male. Serial axial sections at 1.5 mm through a single substantia nigra (SN) A: cartoon showing key anatomical structures based on SP and LFB/CV for borders, LFB/CV for location of pigmented neurons on substantia nigra pars compacta (SNc) and CB for nigrosomes. Pigmented neurons are shown as black dots and it is notable that not all of these fall within the CB-defined nigrosomes. B: LFB/CV. C: Perls stain. D: High field spin-echo MRI. E: SP. F: CB. Anatomical landmarks are in black. Nigrosomes (1–5) are labelled in red. See text for a detailed description of the anatomy and comparison of images.