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. 2004 Aug 9;101(33):12318–12323. doi: 10.1073/pnas.0404924101

Fig. 1.

Fig. 1.

Analysis of temperature-dependent motility and flagellar expression in Listeria stains. L. innocua and L. monocytogenes strains were grown ≈24 h at RT or at 37°C and motility and flagellar expression was analyzed. (A) Two microliters of culture were spotted on low-agar (0.375%) BHI plates and were incubated at 37°C or at RT. (B) Bacterial cultures were stained for flagella and were analyzed microscopically. The percentage (%) of bacteria within a population that possessed at least one visible flagellum is given. (C) Detection of FlaA protein by Western blot. Surface-extracted proteins were separated on an SDS/10% PAGE gel and analyzed by Western blot using a FlaA-specific antibody (lanes 2–9). Lane 1, molecular mass marker (M) with size given in kilodaltons. (D) RNA was isolated from bacteria and analyzed by primer extension for detection of flaA-specific transcripts (lanes 2–7). Lane 1, radiolabeled DNA standard with sizes given in nucleotides.