Fig. 2.

Determination of flaA promoter activity by using lacZ fusions. (A) Schematic representation of flaA promoter-lacZ fusions. Numbers at the beginning of each line indicate the number of base pairs upstream of the flaA translational start site included in each construct. The –35 and –10 promoter elements and the flaA transcriptional start site (10) are indicated as boxes or an arrowhead, respectively. (B) lacZ fusions were introduced in single copy onto the chromosome of EGDe and β-galactosidase activities were determined from cultures grown at 30°C (light shaded bars) or 37°C (dark shaded bars). (C) Same as in B with lacZ fusions integrated into strain 10403S. β-galactosidase activities represent the means plus SD of four individual experiments.