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. 2016 Nov 1;88(23):11498–11503. doi: 10.1021/acs.analchem.6b02677

Figure 3.

Figure 3

P-EIM visualization of subcellular responses to GPCR activation. (a) The bright field image shows the HeLa cell locations, while differential SPR images show the spatial-temporal patterns of subcellular responses to GPCR activation, at t = 3, 18, and 240 s after histamine treatment. Red dotted lines outline the cells in the bright field and SPR images. (b) A typical whole cell SPR response profile to 6 μM histamine triggered GPCR activation in HeLa cells. Arrows P1 to P3 indicate times corresponding to SPR images shown in (a). (c) SPR response profiles of four different subcellular regions within a single cell that are marked by dotted lines with the corresponding colors in the bright field image and by dashed lines with black colors in SPR images in (a).