Skip to main content
. 2004 Aug 4;101(33):12381–12386. doi: 10.1073/pnas.0402868101

Fig. 1.

Fig. 1.

Auxin does not induce mass-shifting modifications within domain II. (a and c) MALDI/TOF MS spectra of trypsinized GST-AXR2dII exposed to plant extract without (a) and with (c) added auxin (10 μM NAA) (n = 5). The 1,108-Da peak corresponding to the major part of the domain II degron and the derivative peak at +15.99 Da are indicated. (b) MALDI/TOF MS spectrum of trypsinized GST-AXR2dIIPP-AA (both prolines replaced by alanines). The new 1,056-Da peak corresponding to the major part of the mutant domain II degron is marked. For comparison, the positions at 1,056 + 15.99 Da, 1,108 Da, and 1,124 Da are indicated (n = 2). n, the number of independent replicates.