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Formation of multilayer scaffold system. (A) Schematic representation of the gradient scaffold. (B) Gross image of the gradient scaffold. (C) Confocal image of a vertical section of the gradient scaffold indicating presence of the distal layer containing the PLGA microspheres (blue), underlying porous scaffold structure (green), and fibrin (red) loaded within the pores. Scale bar is 100 μm (with permission from [20]). PLGA, poly (lactic-co-glycolic acid).
