FIG. 7.

Electrophilic activation of Nrf2 reduces ROS in Tak1-deficient intestinal epithelium. (A, B) Control (Tak1^flox/flox Tnfr1^−/−) and TAK1^IE-IKO Tnfr1^−/− mice were fed with BHA-containing food from 1 week before injection of tamoxifen for 3 consecutive days, and the small intestine was harvested at day 3 (B) or 7 (A). Protein extracts from the intestinal epithelium were analyzed with immunoblotting (A). Each lane represents an individual animal. The immunoblot shown is the same exposure film, and unnecessary lanes were removed. Fresh frozen sections without any fixation were stained by CM-H₂DCFDA (B). Scale bars, 50 μm. ROS, reactive oxygen species. To see this illustration in color, the reader is referred to the web version of this article at www.liebertpub.com/ars